83-5975-31　PNAd (Peripheral Node Addressin) (Biotin) 500ug　046111

特徴

• This antibody recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1).
• This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250kD.
• It has been identified that GlcNAc-6-SO4 sulfation contributes to PNAd binding and the core 1 B1,3-N-acetylglucosaminyltransferase is required for the generation of the PNAd epitope.
• PNAd is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia.
• The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues.
• This antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (frozen/paraffin): 5-10ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.