H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-a (mixed linage kinase-like mitogen activated protein triple kinase a) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：rat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human histone H3, phosphorylated at Ser28
Clone # USB：HTA28
Isotype：IgG2a,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa23-35 of human Histone H3 conjugated to KLH
