83-5975-27　CD43 (CD43 Antigen, Galactoglycoprotein, GALGP, GPL115, Leukocyte Sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN) (Biotin) 200ug　046107

特徴

• CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites.
• Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified.
• The 115kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells.
• It does not present on resting B cells and erythrocytes.
• While the 130kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells.
• It has been reported that CD43 binds to CD54 and Siglec-1.
• CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
• This antibody reacts with the activation-associated glycoform of CD43.
• The epitope recognized by this monoclonal antibody is also shared with desialylated CD45RB.
• This antibody is useful for differentiation of effector CD8 T cells and memory T cells.
• Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volumeImmunohistochemistry: acetone-fixed frozen sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.