High affinity IgE receptor (FceRI) plays a key role in IgE-mediated allergic immune response.
FceRI is a tetrameric receptor complex, which is composed of one a-subunit (FceRIa), one B-subunit, and two y-subunits.
FceRIa directly binds IgE with high affinity, while the B- and y-chains are responsible for mediating intracellular signals.
FceRIa is a 50kD transmembrane protein with Ig superfamily structure.
It is primarily found on mast cells and basophils.
Further studies have indicated that FceRIa is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5ulg/10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human FceRIa. Species Crossreactivity: Baboon, Cynomolgus and Rhesus Monkey. Has been reported to bind the receptor even in the presence of IgE.
