CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120-220kD, mucin-like type I transmembrane glycoprotein.
CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin).
The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 5ul/10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human CD162.
Clone # USB：KPL-1
Isotype：IgG1,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：PSGL-1 transfected murine 300.19 pre B-cell line.
