It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b.
CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers).
CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation.
Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Applications:Suitable for use in Flow Cytometry.
Though not tested, Immunohistochemistry (frozen sections) use has been reported in literature (ref.
6 and 7).
Other applications not tested.
Recommended Dilution:Flow Cytometry: ≤0.5ug labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes the alpha chain of human CD8. Species Crossreactivity: cynomolgus monkey and rhesus monkey. Species sequence homology: African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
