CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily.
It is transiently expressed on activated T cells.
CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells.
Regulatory T cells express high levels of CTLA-4.
CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization.
Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC).
CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Applications:Suitable for use in Flow Cytometry.
Not suitable for use in Immunohistochemistry of paraffin-embedded tissue sections.
Other applications not tested.
Recommended Dilution:Flow Cytometry (intracellular immunofluorescent staining): ≤0.25ug/10e6 cells in 100ul.
Membrane permeabilisation is required
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes human CD152.
Clone # USB：BNI3
Isotype：IgG2a,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
