Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle.
Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells.
Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species.
Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction.
Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/ml using lysates of human heart tissue, mouse heart tissue, rat heart tissue, and human liver tissueImmunohistochemistry: 3-25ug/m in immersion fixed paraffin-embedded sections of human heart ELISA (detection): using 045953 as captureOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography from cell culture supernatant.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in 200ul sterile PBS.
Specificity：Recognizes human Myoglobin. Species Crossreactivity: mouse and rat
Clone # USB：2269B (recomb)
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified human Myoglobin antigen from human heart
