83-5974-75　Adiponectin (Acrp30, GBP-28, apM1, AdipoQ) (Biotin) 500ug　045949

特徴

• Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans.
• Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal.
• The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin).
• This globular domain resembles the structure of the complement C1q and the TNF-α superfamily.
• Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms.
• Females generally have higher HMW than males in proportion and absolute amounts.
• Adiponectin has two receptors (adipoRs)-1 and -2.
• AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver.
• AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA (detection): 0.05-0.2ug/ml.
• To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000-156 pg/ml are recommended for each ELISA plate
• Optimal dilutions to be determined by the researcher.
• Matched Pair:Detection: 045949Capture: 045948
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.