Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans.
Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal.
The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin).
This globular domain resembles the structure of the complement C1q and the TNF-α superfamily.
Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms.
Females generally have higher HMW than males in proportion and absolute amounts.
Adiponectin has two receptors (adipoRs)-1 and -2.
AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver.
AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA (capture): 0.5-2ug/ml.
To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000-156 pg/ml are recommended for each ELISA plate
Optimal dilutions to be determined by the researcher.
Matched Pair:Detection: 045949Capture: 045948
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human Adiponectin.
Clone # USB：62-9
Isotype：IgG2a,k
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Adiponectin.
