CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha).
This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors.
CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer.
Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul reagent/100ul of whole blood or 10e6 cells in a suspensionOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human CD123. 045940 does not inhibit IL-3 binding to its receptor.
Clone # USB：6H6
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
