p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4).
Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified.
Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5).
MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.
Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK.
This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9).
SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry and Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight
Immunoprecipitation: 1:400Immunohistochemistry (paraffin): 1:400-1:1600Immunofluorescence (IC): 1:800-1:1600Flow Cytometry (Fixed/Permeabilized): 1:200-1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human p38 MAPK only when phosphorylated at Thr180 and Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity: mouse, mink, rat, monkey, porcine and S. cerevisiae
Clone # USB：D3F9
Isotype：IgG
Calc Applications Abbrev：FC IC IF IHC IP WB
Calc Crossreactivity：Hu Mk Mo Po Rt Ye
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Species sequence homology (100%): hamster, chicken, zebrafish and bovine
