Influenza A viruses belong to the Orthomyxoviridae family and encapsidates the negative strand viral RNA that protects it from the action of nucleases.
Its envelope membrane is reported to carry hemagglutinin, neuraminidase, and the ion channel protein M2.
Hemagglutinin (HA) binds to the sialic acid present on the surface of its target host cells and the cell membrane then engulfs the virus through endocytosis and forms endosome.
Following virus replication in host cell, neuraminidase removes sialic acid from infected cell surfaces and newly made viruses are released to infect more cells.
Influenza A virus HA contains an antigenically variable globular head domain that has the receptor-binding site (RBS) for viral attachment and a more conserved stem domain that mediates fusion of viral and cell membranes in the endosome.
Clone rFluA-20 displays high binding breadth and affinity to HA timers belonging to group 1 and group 2 viruses with EC50 in the range from 5 to 142 ng/mL.
It is shown to bind with most HA from H1, H2, H3, H5, and H7 subtypes with high affinity (KD < 100 nM).
It interacts with a novel epitope on the non-RBS side of the 220-loop and the adjacent 90-loop.
(Ref.: Bangaru, S., et al.
(2019).
Cell.
177(5); 1136-1152).
Applications:Suitable for use in ELISA and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA: Various (titration, starting at 1mg/ml) detected recombinant Influenza A H1N1 hemagglutinin
Neutralization: displayed neutralization activity in plaque assay with H3N2 A/Hong Kong/1/1968 virus causing a 3.8-fold reduction in the plaque size compared to the control.
(Bangaru, S., et.
al.
(2019).
Cell.
177(5):1136-1152.e18)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes Influenza A virus hemagglutinin in multiple strains.
Clone # USB：rFluA-20 (recomb)
Isotype：IgG1
Calc Applications Abbrev：E N
Immunogen：PBMCs from human donor, who was subjected to Flu vaccine multiple times, were immortalized and used generate human hybridoma cell lines secreting human mAbs. Epitope: Hemagglutinin head domain; non-RBS side of the 220-loop and the adjacent 90-loop
