Herpes Simplex Virus envelope Glycoprotein D (UniProt: Q69091) is also known as gD.
The envelope of herpes simplex virus (HSV) is complex and contains at least 10 virus-encoded glycoproteins, some of which mediate virus entry into the host cell.
HSV virus enters the cell by first fusing its envelope with a membrane of the host cell.
Five viral envelope glycoproteins are reported to be involved in this entry process.
Binding of glycoprotein D of HSV to a cell surface receptor is required to trigger membrane fusion during entry into host cells.
Glycoproteins C and B promote attachment by interacting with cell surface proteoglycans and then glycoprotein D binds to the herpes virus entry mediator (HVEM) receptor that initiates a process that ultimately leads to glycoprotein B-mediated membrane fusion.
Glycoprotein D is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off.
It is a homodimeric protein that contains two zinc-binding sites (aa 64 and 264).
The ectodomain region of glycoprotein D contains three sites for the addition of N-linked oligosaccharides (N-CHO) and six cysteine residues arranged into three disulfide bonds.
During virion morphogenesis, glycoprotein D probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs.
(Ref.: Whitbeck JC et al.
(1997).
J.
Virol.
71(8); p.
6083 6093; Nicola, AV et al.
(1998).
J Virol.
72(5): 3595-3601; Giovine, PD et al.
(2011).
PLos Pathog.
7(9); e1002277).
Applications:Suitable for use in Immunocytochemistry, Western Blot and Inhibition.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000 detected Herpes Simplex Virus Glycoprotein D in lysates from VERO cells transfected with HSV-1
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes glycoprotein D of Herpes Simplex virus.
