Fibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human.
Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts.
Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region.
Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively.
Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types.
Cellular fibronectin is deposited as fibrils in the extracellular matrix.
Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3.
The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD).
Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.
Applications:Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1:50 detected Fibronectin in human kidney and human cerebellum tissues
Western Blot: 1ug/ml detected Fibronectin in 10ug of human thymus tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Fibronectin. Species Crossreactivity: mouse and porcine
