CA19-9 (sialylated Lewis a antigen) is a serum tumor marker for pancreatic cancer diagnosis.
It is also used for monitoring therapy in patients with pancreatic adenocarcinoma.
CA19-9 is synthesized by normal pancreatic and biliary ductal cells and by gastric, colon, endometrial and saliva epithelia.
In a normal patient, it is present in small amounts in serum.
The plasmatic levels of CA19-9 are greatly increased in neoplastic disease.
Applications:Suitable for use in Flow Cytometry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25ug/10e6 cells using Capan-2 human pancreatic adenocarcinoma cell lineImmunocytochemistry: 8-25ug/ml using immersion fixed Capan‑2 human pancreatic adenocarcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography from hybridoma culture supernatant
Form:Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:Recognizes human CA19-9
Clone # USB:23K4
Isotype:IgG2a
Calc Applications Abbrev:FC IC
Calc Crossreactivity:Hu
Immunogen:Human CA19-9 from Capan-2 human pancreatic adenocarcinoma cell line