83-5974-22　Neurophysin 1 100ug　045891

特徴

• Neurophysins are linear cysteine-rich proteins containing 93-95 amino acids.
• Two types of neurophysins, NPI and NPII are found in most mammalian species and they serve as carriers of oxytocin (OT) and vasopressin (AVP), respectively.
• Each NP and its associated hormone is synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or VAP-NPII (UniProt P01186) encoded by the Avp gene in rat species.
• Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII).
• OT is a potent hormone and neurotransmitter secreted within the pituitary gland.
• OT exerts its biological activity through its receptor OXTR (also known as OTR).
• OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders.
• VAP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood.
• VAP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and RIA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/ml detected Neurophysin 1 in 10ug of rat pituitary tissue lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.