Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen.
Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism.
The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia.
In the central nervous system, Apo-E is produced by astrocytes and microglia.
The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity.
Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance.
Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
Applications:Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Flow Cytometry (intracellular immunofluorescent staining): 5ul/10e6 cells in 100ulOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.
Clone # USB：2B16 (E6D7)
Isotype：IgG1,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
