IgM is the first immunoglobulin made by B cells in the immune response.
Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
Note: due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Applications:Suitable for use in ELISA and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (immunofluorescent staining): ≤0.5ug/10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes both soluble and membrane human immunoglobulin M (IgM). Does not react with other Ig isotypes Species Crossreactivity: African Green, Baboon, Cynomolgus, Rhesus
