Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide.
Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1).
These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2).
There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3).
HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock.
HO-2 is a constitutive isoform which is expressed under homeostatic conditions.
HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4).
It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7).
Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4)
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence Other applications not tested.
Recommended Dilutions:Immunofluorescence (IC): 1:100Western Blot: 1:1000Optimal dilutions to be determined by researcher
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
Specificity：Recognizes rat heme oxygenase-1 at ~32kD. Does not cross-react with HO-2. Species Crossreactivity: mouse and human
