The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes.
This regulated transport is important for appropriate protein localization, secretion, and signal transduction.
Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains.
Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1).
The Golgi cisternae are stacked and linked laterally to form a ribbon.
GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly.
These lateral fusion events allow for uniform distribution of Golgi enzymes.
GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport.
GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel.
Inappropriate hERG localization may be an underlying cause in Long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia.
Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4.
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot.
Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total mouse GM130. Nonspecific staining has been observed in mouse testis by immunofluorescence. Species Crossreactivity: rat
Clone # USB：E9Z6S
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Pro77 of mouse GM130
