83-5973-99　HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B + C, MHC HLA ABC) (Low Endotoxin, Azide Free) 1mg　H6098-39RX

特徴

• The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections.
• In humans, this complex is referred to as the human leukocyte antigen (HLA) region.
• There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.
• These proteins are found on the surface of almost all nucleated somatic cells.
• HLA-ABC antigen is present on most nucleated cells of humans and old world monkeys.
• HLA Class 1 Antigen ABC is a monomorphic determinant of human Class I HLA molecules (HLA-A,B,C) associated with b2-microblogulin.
• It is expressed at the surface of human lymphoblastic B cells.
• Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC).
• HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C.
• HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD).
• The smaller beta chain bears homology with immunoglobulin molecules.
• The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus.
• This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues.
• HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor.
• HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells.
• Applications:Suitable for use in Blocking (6,7), Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation (2), Inhibition of NK cell-mediated lysis (10), and Activation (8,9).
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence: ≤2ug/10e6 cells in 100ul volume or 100ul whole blood
• Western Blot: Non-reducing (3)Immunohistochemistry: Acetone-fixed frozen sections (4,5)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.