The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections.
In humans, this complex is referred to as the human leukocyte antigen (HLA) region.
There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.
These proteins are found on the surface of almost all nucleated somatic cells.
HLA-ABC antigen is present on most nucleated cells of humans and old world monkeys.
HLA Class 1 Antigen ABC is a monomorphic determinant of human Class I HLA molecules (HLA-A,B,C) associated with b2-microblogulin.
It is expressed at the surface of human lymphoblastic B cells.
Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC).
HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C.
HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD).
The smaller beta chain bears homology with immunoglobulin molecules.
The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus.
This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues.
HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor.
HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5ul/10e6 cells in 100ul volume or 100ul whole blood
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human HLA-A,B,C. Recognizes a non-polymorphic epitope. Species Crossreactivity: chimpanzee, baboon, African Green monkey, Cynomolgus monkey, Rhesus monkey, bovine and feline.
