CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII.
It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells.
There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing.
CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation.
The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: ≤ 0.5ug/10e6 cells in 100ul volumeOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes human CD32. Recognizes both CD32A and CD32B.
