83-5973-80　Tau (Tau Protein, Microtubule-associated protein tau, MAPT) 100ul　045848

特徴

• MAP-τ is a low molecular weight member of the microtubule associated protein or MAP family.
• Several serious human diseases are associated with accumulations of MAP-τ, usually referred to as tau protein, most notably the neurofibrillary tangles of Alzheimer’s disease.
• Accumulations of tau in neurons are also characteristic of chronic traumatic encephalopathy, Pick’s disease and several other neurodegenerative diseases.
• Together these disorders are known as “tauopathies”.
• The single mammalian tau gene produces at least 9 different proteins by alternate transcription.
• In the central nervous system 6 isoforms ranging from 48-67kD by SDS-PAGE predominate, though larger isotypes are seen mostly in the peripheral nervous system.
• The tau molecules are very heavily charged and run on SDS-PAGE much more slowly than predicted from their real molecular size.
• For example the smallest human tau isotype runs at 48kD on SDS-PAGE but the real molecular weight is 32kD.
• Tau proteins are substrates for ser/thr phosphorylation and other post-translational modifications.
• Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000Immunohistochemistry: 1:10,000Immunofluorescence (IC): 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.