83-5973-79　mCherry 100ul　045847

特徴

• mCherry protein is derived from a natural product, DsRed, originally isolated as a red fluorescent protein from the coral of the genus Discosoma (1).
• As with other natural fluorescent proteins of Cnidarians (jelly fish, sea anemones and corals), the natural form of the protein forms stable tetramers in vivo.
• DsRed was engineered to improve its spectral properties and also prevent multimerization in the lab of Roger Tsien, where much work on fluorescent proteins was performed (2).
• Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587nm and emission maximum at 610nm (3).
• The protein is widely used as a fluorescent tracer in transfection, transgenic, photobleaching and FRET type experiments.
• The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kD protein isolated originally from the jellyfish Aequoria victoria (4).
• The mCherry protein is similar in size and general structural properties to GFP (5,6), but, obviously, produces a red rather than a green fluorochrome.
• 045847 can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected cells.
• Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:500 Western Blot: 1:2000Immunohistochemistry: 1:500, specifically detected the soma and axons of mCherry positive neurons in the cerebellum, as expected for this model.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.