83-5973-74　Methyl-CpG Binding Protein 2 (MeCP2) 100ul　045834

特徴

• Methyl-CpG Binding Protein 2 (MeCP2) is widely expressed in tissues and particularly heavily in neurons.
• It is a nuclear protein which was found to bind methylated cytosines in CpG islands in DNA, but which may also bind hydroxymethyl cytosine residues (1-3).
• Methyl and hydroxymethyl cytosines are generally found in regions of DNA regulating the expression of specific genes and MeCP2 is thought to function as a gene regulator, though it may have other functions.
• The MeCP2 protein contains one methyl-CpG binding domain and two AT hook domains, highly basic peptides which bind AT rich DNA sequences.
• The MeCP2 gene, MECP2 is located on the X-chromosome and loss of function mutations of the gene cause Rett syndrome, a relatively rare neurodevelopmental, autistic disorder which presents almost exclusively in females, since similar mutation of MECP2 in males is almost invariably lethal (3).
• Rett patients are apparently normal at birth but develop neurological problems over the period when extensive synaptogenesis is occurring, which coincides with the expression of high levels of MeCP2 in normal brain.
• The Rett brain shows smaller more densely packed neurons with less extensive dendritic branching (2).
• Duplication of the MECP2 gene was found to be the cause of a different neurodevelopmental disorder referred to a MECP2 duplication syndrome (4).
• Levels of MeCP2 normally alter dynamically and the protein is regulated by phosphorylation on multiple sites (5-7).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:5000-1:10,000Immunohistochemistry: 1:1000-1:5000Immunofluorescence (IC): 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.