Phosphatase and tensin homolog on chromosome ten (PTEN) is a ubiquitously expressed tumor suppressor which, in the cytoplasm, antagonizes the PI3K signaling pathway through its lipid phosphatase activity.
Mutations of this gene are an important step in the development of many cancers since PTEN regulates essential cellular process such as growth and survival.
PTEN is also found in the nucleus, and studies have demonstrated that its presence in the nucleus is regulated by SUMOylation and phosphorylation.
Also, studies have showed that cells lacking nuclear PTEN are more sensitive to DNA damage.
Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation: 1:100Western Blot: 1:1000Immunohistochemistry: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes PTEN at C-terminal. Species Crossreactivity: human, monkey and rat.
Clone # USB：6H2.1
Isotype：IgG2a,k
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mk Rt
Immunogen：C-terminal peptide corresponding to human PTEN.
