83-5973-62　Granzyme B (FITC) 100Tests　045822

特徴

• Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells.
• Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity.
• Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases.
• Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 4ul/100ul of whole blood or 10e6 cells in a suspensionOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.