Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human.
Collagen is the major component of the extracellular matrix (ECM) and forms the fibrils of tendons, ligaments, and bones.
Type I collagen consists of two alpha I chains and one alpha 2 chain.
Alpha-1 type I collagen is initially produced as a 1464-amino acid prepro-form with a signal peptide sequence (a.a.
1-22) and two propeptide sequences (a.a.
23-161 and a.a.
1219 –1464), the removal of which yields the mature alpha-1(I) chain.
The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a.
179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a.
162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a.
1193-1218; numbering based on the prepro-form).
Collagen can be extracted from tissue via either enzymatic or non-enzymatic means.
Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin.
On the other hand, collagen preparations obtained with non-enzymatic means (e.g.
by acid extraction) have the intact telopeptides at both ends.
Applications:Suitable for use in Immunohistochemistry, ELISA, Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1:50 using heat-induced epitope retrieval (HIER)
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride. No preservative added.
Specificity：Recognizes carboxy-terminal pro-peptide of human collagen type I. Does not stain mature collagen fibers in tissue, but rather is localized intracellularly in cells producing pro-collagen I. Species Crossreactivity: mouse, rat and guinea pig
Clone # USB：PCIDG10
Isotype：IgG1,k
Calc Applications Abbrev：E FC IC IHC
Calc Crossreactivity：Gp Hu Mo Rt
Immunogen：Human pro-collagen I. Epitope: C-terminal propeptide region. Species sequence homology: bovine
