Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25kD protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human.
SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events.
Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion.
SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane.
SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.
Applications:Suitable for use in Immunohistochemistry, Western Blot and Electron Microscopy.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml detected SNAP-25 in 10ug of human brain tissue lysate
Immunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride. No preservative added.
Specificity：Recognizes both SNAP-25 isoforms A & B. Reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity. Species Crossreactivity: bovine, mouse, guinea pig, monkey and rat.
Clone # USB：SP14
Isotype：IgG1,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Gp Hu Mk Mo Rt
Immunogen：Human brain proteins immunoprecipitated with EP10. Epitope: N-terminal half
