Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein.
The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start.
The spindle checkpoint becomes triggered when the chromosome(s) are unaligned.
Mad2 then binds to the anaphase promoting complex (APC) and CDC20.
This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml detected Mad2 in 10ug of HEK293 cell lyate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Mad2.
Clone # USB：AS55-A12
Isotype：IgG2b
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：His-tagged recombinant protein corresponding to human Mad2.
