The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments.
Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells.
γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers.
Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration.
These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunofluorescence (IC): 1:1000-1:2000Immunohistochemistry (paraffin): 1:50-1:200Flow Cytometry (fixed/permeabilized): 1:400-1:1600Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：chicken
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total alpha-tubulin protein. Species Crossreactivity: mouse, rat and monkey
Clone # USB：DM1A
Isotype：IgG1
Calc Applications Abbrev：FC IC IF IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Full-length chicken alpha-tubulin purified from brain extracts, and recognizes residues surrounding Val440 of alpha-tubulin protein.
