Manufactured using Recombinant Platform with variable regions (i.e.
specificity) from the hybridoma MCS6-27
Botulism, an infection of the bacterium, which is commonly spread through contaminated food, can be a fatal disease, and symptoms include a gradual flaccid paralysis.
Paralysis is caused by the internalization by endocytosis of the toxin in specific axons, mediated by the attachment of the heavy chain of the protein to axon surface.
Once inside the axon, BoNT/B acts by cleaving SNARE proteins, such as VAMP, which are involved in the docking and fusion of neurosecretory vesicles.
This prevents the release of the neurotransmitter acetylcholine, resulting in paralysis of the muscles.
045756 is able to capture BoNT/B at SDS concentrations between 0 and 0.4 mM, and can be used to screen food samples for botulinum toxin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
Specificity：Recognizes Clostridium botulinum Neurotoxin. Binds to the Hc and H5 fragments of BoNT/B, and the binding epitope is located in the heavy chain, between amino acids E859 and E1291.
Clone # USB：MCS6-27 (recomb)
Isotype：IgG1,k
Calc Applications Abbrev：E WB
Immunogen：Recombinant BoNT/B fragments expressed in E. coli as GST-fusion proteins.
