Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution.
The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation.
PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system.
045744 binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules.
It is suitable for sensitive ELISA applications 045744 can be used as a capture antibody.
Note: Tween-20 should be used in wash buffers for this antibody combination.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.
Recommended Dilutions:ELISA: 5ug/ml detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Highly Purified
Purity：Purified by Ion-exchange chromatography
Form：Supplied as a liquid in PBS, 0.04% sodium azide.
Specificity：Recognizes proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Clone # USB：AGP4
Isotype：IgM,k
Calc Applications Abbrev：E IHC WB
Immunogen：Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
