Caspase-8 (Cysteine-aspartic acid protease 8/Casp8; also MCH5 and FLICA) is a 28kD member of the peptidase C14A family of enzymes.
It is known as the initiating caspase for the apoptotic cascade.
Caspase-8 acts on procaspases-3, 4, 6, 7, 9 and 10, in addition to c-FLIPL and procaspase-8 itself.
Human procaspase-8 alpha 4 is a 58kD (predicted), 496 amino acid (aa) protein and contains two N-terminal death domains plus a catalytic site that utilizes His334Gly335 plus Cys377.
Following death domain-containing receptor activation, proteolysis generates a 28 kDa heterodimer.
This includes a p18 subunit (aa 234-391; or 217-374 of the 8a/standard form) noncovalently linked to a p10 subunit (aa 402-496; or 385-479 of the 8a/standard form).
Association with another p18/p10 heterodimer generates active caspase-8.
There are two common procaspase-8 isoform variants.
One 53kD isoform (8b) shows a deletion of aa 103-134, while a second 55kD (8a) isoform shows a deletion of aa 103-134 with a 15 aa insertion after Lys183.
The p18 and p10 subunits are 68% and 82% aa identical, human to mouse, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1mL sterile PBS.
Specificity：Recognizes human Caspase-8 precursor in Western blots and a 42kD doublet generated during apoptosis.
Clone # USB：23H53
Isotype：IgG2a
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa234-391 from human Caspase-8, expressed in E. coli.
