This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family.
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.
This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10.
It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Alternative splicing of this gene results in two transcript variants that encode the same protein.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000..
A band is seen at ~32kD for the inactive form of Caspase 3 and ~17-22kD for the active form of Caspase 3Immunohistochemistry (frozen and paraffin): 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in PBS, pH 7.6, 0.09% sodium azide.
Specificity：Recognizes the full length 32kD human caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members.
Clone # USB：4-1-18
Isotype：IgG2a,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Full-length recombinant protein corresponding to human Caspase 3. Epitope: large subunit and proform
