83-5973-14　CD44 (HCAM, Pgp-1, Heparan Sulfate Proteoglycan) 100ul　045734

特徴

• CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM).
• CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2).
• Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response.
• Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth (1-3).
• CD44 interacts with ezrin, radixin, and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6).
• CD44 is constitutively phosphorylated at Ser325 in resting cells.
• Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:50Immunofluorescence (IC): 1:400Immunoprecipitation: 1:50Flow Cytometry (Fixed/Permeabilized): 1:100Flow Cytometry (Live): 1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.