Nesprin-2 (SYNE-2) is also known as Nuclear envelope spectrin repeat protein 2 or Nucleus and actin connecting element protein, and it functions to network organelles and the actin cytoskeleton in order maintain subcellular spatial organization.
Nesprin-2 connects nuclei to the cytoskeleton by interacting with the nuclear envelope and cytoplasmic F-actin, and is required for centrosome migration to the apical cell surface during early ciliogenesis.
Nesprin-2 is widely expressed, with high levels seen in kidney, adult and fetal liver, stomach and placenta.
Defects in SYNE2 are responsible for Emery-Dreifuss muscular dystrophy type 5 (EDMD5).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 1:50 detected Nesprin-2 in HepG2 and COS-7 cells
Western Blot: 0.5ug/ml detected Nesprin-2 in 10ug of COS-7 cell lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Nesprin 2. Species Crossreactivity: mouse
Clone # USB：K20-478
Isotype：IgG2b,k
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：His-tagged recombinant protein corresponding to human Nesprin-2.
