Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.
Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004; Logani et al., 2003; Murray et al., 2004; Roy et al., 2011).
CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004).
Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005).
Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011; pmid: 21693612).
Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry; for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014; pmid: 24326460).
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：human
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin-300.
Specificity：Recognizes human Cytokeratin 7. Reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.
