Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB.
PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates.
PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β.
PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain.
Their ligand, PDGF, is a mitogen for connective tissue and glial cells.
CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice.
PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
Applications:Suitable for use in Flow Cytometry.
Immunohistochemistry reported in literature (ref.
4).
Other applications have not been tested.
Recommended Dilutions:Flow Cytometry: ≤0.25ug/10e6 cellsImmunohistochemistry: frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes mouse CD140a, the alpha chain of PDGF receptor.
Clone # USB：APA5
Isotype：IgG2a,k
Calc Applications Abbrev：FC IHC
Calc Crossreactivity：Mo
Immunogen：Mouse PDGFR-alpha-hIgG1 recombinant fusion protein
