It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages.
CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins.
CD61 is also a member of the integrin family known as gpIIIa or β3 integrin.
It is a 110 kD common β subunit of CD51/CD61 or CD41/CD61 complex.
CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation.
Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1.
CD51/CD61 also mediates cell-cell adhesion via interaction with CD31.
CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components.
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: ≤0.5ug/10e6 in 100ul Immunoprecipitation: Reported see reference 5Immunohistochemistry: Reported see reference 5Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography. Endotoxin: ≤ 0.01EU/ug (<0.001ng/ug) (LAL)
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. For antibody with azide see C2405-17U.
