83-5972-98　Transferrin Receptor (CD71) 100ul　045717

特徴

• Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis (1).
• Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor.
• The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome.
• Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron (2).
• The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor (2).
• Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state.
• The interaction between receptor and ligand has been studied in detail.
• The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process (3).
• Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE).
• HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake (4,5).
• In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes (6).
• Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number of arenaviruses and at least one retrovirus (7,8).
• Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor (9,10).
• Applications:Suitable for use in Western Blot and Immunofluorescence/ Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:200-1:800Western Blot: 1:1000Optimal dilutions to be determined by the researcher
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.