Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells.
EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1).
Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue.
Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Fixed/Permeabilized): 1:400-1:1600Flow Cytometry (Live): 1:400-1:1600Immunohistochemistry (paraffin): 1:500Western Blot: 1:1000Immunofluorescence (IC): 1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human EpCAM protein.
Clone # USB：VU1D9
Isotype：IgG1
Calc Applications Abbrev：FC IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：NCI-H69 small cell lung carcinoma cells.
