83-5972-94　CHIKV (Chikungunya Virus) 100ul　045714

特徴

• Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes.
• Immune compromised individuals may face serious complications, including encephalitis and mortality.
• CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K.
• The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion.
• The ectodomain E1 protein consists of three domains known as D1, DII, and DIII.
• The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker.
• Both E1 and E2 proteins are responsible for virus entry into host cells.
• The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions.
• Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating.
• 045714 can bind strongly to native CHIKV surface and potently neutralize virus replication (Ref.: Marsinoul, P., et al.
• (2014).
• Virology 464-465; 111-117; Lam, S., et al.
• (2015).
• mAbs 7(6), 1178-1194).
• Applications: Suitable for use in ELISA, Immunocytochemistry, Neutralization and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1:100 detected Chikungunya virus in BHK cells infected with Chikungunya virus.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.