Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3).
Lamins have been subdivided into types A and B.
Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA.
Lamin A and C are cleaved by caspases into large (41-50kD) and small (28kD) fragments, which can be used as markers for apoptosis (4,5).
Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8).
Lamin B1 is also cleaved by caspases during apoptosis (9).
Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
Applications: Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Lamin B1 protein. This antibody recognizes the 25kD lamin B1 C-terminal cleavage product produced during apoptosis. Species Crossreactivity: mouse and rat
Clone # USB：D4Q4Z
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Leu118 of human lamin B1. Species sequence homology: monkey and porcine.
