Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species.
BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309).
A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127.
It is expressed by cells of myeloid origin and by stromal cells.
Two isoforms of BAFF have been described that are produced by alternative splicing.
Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1.
BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA.
It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation.
Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE).
Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells.
Applications: Suitable for use in ELISA, Activity Assays and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA: direct Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS. No preservative added.
