Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E.
Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1).
Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3).
Multiple 4E-BP1 residues are phosphorylated in vivo (4).
While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of mouse 4E-BP1 when phosphorylated at Ser65. Species Crossreactivity: human and monkey
Clone # USB：174A9
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1. Species sequence homology: chicken
