This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Applications:Suitable for use in Flow Cytometry, Western Blot, Activating, Agonist and Functional Assays,.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Application Notes:FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses.
For instance, the rat IgG2a version of this antibody (592462) was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumor (Luheshi et al., 2016; pmid: 26918344).
Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015; pmid: 26351680).
Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015; pmid: 25924063).
On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo.
Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996; pmid: 8885865).
Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018; pmid: 30097293).
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
