Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV).
The 47kD E1 glycoprotein mediates viral membrane fusion during CHIKV infection.
The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface.
The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells.
The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:Recognizes Chikungunya Virus E1
Clone # USB:23H45
Isotype:IgG2b
Calc Applications Abbrev:WB
Immunogen:Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1, Tyr810-Gly1224